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CellGenix® GMP SCGM Stem Cell Growth Medium
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StemSpan™ SFEM II Human Hematopoietic Cell Culture Medium
WebFeb 2, 2024 · Methods: RPMI-1640, stem cell growth medium (SCGM), NK MACS and TexMACS were used as culture mediums. Activated and expanded NK cells (NKAE) were obtained by coculturing total peripheral blood mononuclear cells (PBMC) or CD45RA + cells with irradiated K562mbIL15-41BBL or K562mbIL21-41BBL. Fold increase, NK cell … Webwere activated in CellGenix® GMP Stem Cell Growth Medium (SCGM, CellGenix, Freiburg, Germany) supple-mented with 10% human serum (Sigma-Aldrich, St. ... cancer cell line) were obtained from the ATCC. Cells were cultured in RPMI 1640 medium (Gibco/Life Tech-nologies, Carlsbad, CA) supplemented with 10% heat-inactivated FBS, … WebJul 5, 2024 · Cells were activated in CellGenix® GMP Stem Cell Growth Medium (SCGM, CellGenix, Freiburg, Germany) supplemented with 10% human serum (Sigma-Aldrich, St. Louis, MO) and the cytokines IL-2 (10 or 100 ng/ml), IL-15 (10 ng/ml), IL-27 (10 ng/ml) (Peperotech, Inc. NJ) and IL-18 (10 ng/ml) (R&D Systems, Inc., MN) under standard … good score in scrabble