Ly49h 3d10
Web15 nov. 2024 · Meanwhile, a modestly but significantly lower percentage of NK cells expressed Ly49I, Ly49A, Ly49D, and Ly49H in SFR KO mice than in WT mice. NKG2D deficiency exerted an additional effect on the expression level of Ly49D, but not Ly49H, Ly49I, and Ly49A (Fig. 3D). We conclude that NKG2D deficiency itself does not affect … Web4 aug. 2015 · WT splenic Ly49H + NK cells were transferred into Ly49H-deficient mice and infected with MCMV. Shown are representative histograms and quantification of mean fluorescence intensity (MFI) for tetramethylrhodamine ethyl ester (TMRE) staining (A), MitoSox Red staining (B), or MitoTracker Green staining (C) in transferred NK cells from …
Ly49h 3d10
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Web(B) NK cells in the spleen according to their expression of CXCR3. (C) As described in the legend to panel B but for Ly49H expression. (D) As described in the legend to panel B but for Ly49C/I expression. (E) As described in the legend to panel B but for NKG2A expression. (F) MFI of NKG2D in NK cells in the spleens of the indicated mice. WebThe 3D10 monoclonal antibody specifically binds to mouse Lymphocyte antigen 49H (Ly-49H; also known as Klra8 or Killer cell lectin-like receptor 8). The 3D10 antibody does not crossreact with related molecules such as Ly-49A, C, D or G2. Ly-49H is a type II transmembrane protein and a member of the Ly-49 C-type lectin multigene family of …
Web28 ian. 2024 · Ly49H positive NK cells expand in the spleen and in the liver during MCMV infection, following the recognition of the viral epitope m157 on infected cells. This proliferative response peaks seven days after infection. We monitored the frequency of Ly49H positive NK cells in the spleen of mice at day-7 postinfection. WebApplications Tested: This 3D10 antibody has been tested by flow cytometric analysis of C57Bl/6 spleen cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can ...
WebSupplemental Experimental Procedures Parabiosis Parabiotic mice were generated as reported (Franklin et al., 2014) using 5- to 6-week-old age- and weight- WebInvitrogen Anti-Ly-49H Monoclonal (3D10), eBioscience™, Catalog # 14-5886-82. Tested in Flow Cytometry (Flow), Immunoprecipitation (IP) and Functional assay (FN) applications. …
Web14 mai 2016 · For animals that are productively infected with MCMV, evaluate the numbers and/or percentage of Ly49H + NK cells in the blood or specific organs (e.g., spleen, liver, or lungs) at various time points post-infection. Typically, time points of interest include day 7 (peak of the effector phase), day 14, and day 21 (the contraction phase), and day ...
WebFITC anti-mouse Ly49H Antibody - Ly49H, also known as Cmv1 and Klra8, is a 28.3 kD protein. It is expressed on NK cells (but not on NKT cells) from C57BL/6 mice (but not … fairyland nswWeb1 ian. 2024 · With respect to the acquisition of adaptive immune features following infection, the interaction between Ly49H + NK cells from C57BL/6 (B6) mice with the m157 viral immunoevasin expressed by MCMV is among the best characterized (), and this system is broadly analogous to the interaction between NKG2C + NK cells with human CMV … fairyland nurseryWeb5 iun. 2008 · The small but significant percentage of 3D10-specific NKT cells suggests that either Ly49H is expressed on NKT cells or, more likely, that a closely related inhibitory … doj abbreviation meaningWeb16 dec. 2024 · Ly49H, NKp46, or NKG2D were, respectively, neutralized using 200 μg mAbs 3D10, 29A1.4, or C7 given intraperitoneally 24 h before infection (80–82). Infected … fairyland nursery chiswickWebPurified anti-mouse Ly49H Antibody - Ly49H, also known as Cmv1 and Klra8, is a 28.3 kD protein. It is expressed on NK cells (but not on NKT cells) from C57BL/6 mice (but not … fairyland new food traysWeb22 sept. 2024 · To investigate the role of T and B cells in adaptive NK cell responses against MCMV infection, we compared Ly49H + NK cell expansion in wild-type (WT) mice versus Rag2 −/− mice, which lack adaptive lymphocytes. Whereas WT mice exhibited a robust NK cell response, peaking ∼7 days post-infection (PI), Rag2 −/− mice showed a diminished … doja cannabis company stock priceWeb18 aug. 2015 · NK Cells Undergo Dynamic Changes in Mitochondrial Quality in Response to Viral Infection In Vivo. WT splenic Ly49H + NK cells were transferred into Ly49H-deficient mice and infected with MCMV. Shown are representative histograms and quantification of mean fluorescence intensity (MFI) for tetramethylrhodamine ethyl ester (TMRE) staining … doja and the weeknd