Nigericin effect on ph
WebOct 5, 1976 · 1. The effect of ionophores on the intralysosomal pH (as estimated from the distribution of a weak acid or base), on the distribution of 42K+ across the lysosomal membrane, and on the intralysosomal degradation of 125I-labelled bovine serum albumin has been studied. 2. Nigericin and X537A equilibrat … 1. WebNigericin has been employed to study anion transport ( Section 2.5) and to modify the pH gradient across energy-conserving membranes. It is often stated that nigericin abolishes ΔpH across a membrane; in fact, the ionophore equalises the K + and H + concentration gradients, with the final ion gradients depending on the experimental conditions.
Nigericin effect on ph
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WebEffect of Trace Levels of Nigericin on Intracellular pH and Acid-Base Transport in Rat Renal Mesangial Cells M.O. Bevensee, E. Bashi, W.F. Boron Department of Cellular and Molecular Physiology, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06520, USA Received: 14 October 1998/Revised: 2 March 1999 Abstract. WebJun 25, 2024 · Nigericin antiports K + and H +, an electroneutral exchange, to collapse only the ∆pH. Valinomycin instead carries only larger cations, not protons, and so collapses the ∆ψ while maintaining the ∆pH ( 15 ). BDQ has been proposed to function as a cationic protonophore ( 11 ).
WebApr 26, 2024 · In three colorectal cancer cell lines (NCI-H747, DLD1, Caco2), growth was optimal near pH 7.4, and the effect of incubation at pH 7.7 varied, with the strongest inhibition of growth in NCI-H747 ... Web3.3 IL-6 cytokine secretion in BMDM of diabetic animals stimulated with LPS and Nigericin. We have also investigated the effect of hyperglycemia concomitant with LPS+Nigericin stimulation to assess the secretion of the pro-inflammatory cytokine IL-6 ().After 2, 4, 6 and 24 hours of incubation, BMDM from diabetic and non-diabetic animals released a more …
WebFeb 10, 2003 · A similar effect on Δψ was observed by the addition of nigericin (Fig. 4E). In both cases, dissipation of the ΔpH resulted in an increase of Δψ. However, in contrast to the effects of benzalkonium or nigericin, adding chloramphenicol or thiamphenicol to MdfA-containing vesicles resulted in a marked dissipation of Δψ (Fig. 4 B and C ... WebJan 3, 2024 · The levels of IL-1β release were close to nigericin, a potent inflammasome activator due to its ability to induce potassium efflux. We found that these activators induced caspase-1 activation and...
WebAcidification of the cytoplasm of Ehrlich ascites carcinoma cells to pH 6.3 arrests DNA synthesis in these cells. Such an effect can be achieved by incubating the cells at pH 6.2 …
WebIn the presence of monensin and nigericin, lysosomal pH levels of cells were adjusted to the pH values of the incubation 2-(N-morpholino ethane sulfonic acid buffers: 4.40, 4.90, and … tpwd flounder seasonWebMar 19, 2003 · Nigericin was diluted to a final concentration of 5 μM from the 10-mM stock solution in absolute ethanol. PIPES buffers were used when the desired pH values were below 6.7. Cells were incubated with the nigericin/high K + buffers at different pH values for 10 min before the collection of pH calibration points. tpwd fishing rulesWebApr 30, 2024 · Nigericin is an antibiotic derived from Streptomyces hygroscopicus that acts as an antiporter of K + /H + and raises the pH of acidic compartments ( 4 ). As early as in … tpwd fish regulationsWebAcidification of the cytoplasm of Ehrlich ascites carcinoma cells to pH 6.3 arrests DNA synthesis in these cells. Such an effect can be achieved by incubating the cells at pH 6.2 or by adding low concentrations of the K+/H+ antiporter, the antibiotic nigericin, at neutral pH. Glucose and anaerobiosis potentiate the nigericin effect. thermostat mitsubishi ecodanWebThe effect of nigericin (1.4 X 10-s M) and varying con- centrations of KC1 on respiration of mitochondria in the presence of p-hydroxybutyrate (10 mM) and aurovertin (3 pg per ml). … tpwd flounder regulations 2022WebApr 11, 2024 · For further exploration the important effect of pyroptosis on the pathogenesis of endometritis and intervention role of TFC, LPS combined with nigericin to induce MEECs was used to establish a pyroptosis model of endometritis cells in vitro. Nigericin, a rapid and potent inducer of cell death, enhances the activation of NLRP3 inflammasome, and ... tpwd form 143Webfew seconds and fully recovers by titrating the pH back to 7.0 (Fig. 1A). The high sensitivity of Chl fluorescence to environmental pH allows us to explore the pH dependence of the NPQ process; note that the ionophore nigericin was added during the pH titration to ensure complete pH equilibrium within the cells (22). The pH thermostat mit display