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Peasy庐-blunt zero cloning kit

WebThe Zero Blunt™ PCR Cloning Kit is offered in a variety of configurations: with One Shot™ TOPO10 Chemically Competent E. coli (K2700-20 and K2700-40) and without competent … WebpEASYpEASY ?-T1 Cloning Kit 目录号:...测序用 M13 F,M13 R,T7 promoter 引物测序,进行 序列 分析。 注意! 1. ... pGM- T载体全序列 pGM-T载体全序列pGM- T 载体序列 pGM- T 载体 是在60碱基处用EcoRV切开 (星号处),然后在3’端加T形成的 (加入的T未包含在此... pEASY T 载体 pEASY T载体_基础医学_医药卫生_专业资料。加强零背景载体的监督, 分子生物学家...

Zero Blunt™ PCR Cloning Kit - Thermo Fisher Scientific

WebZero Blunt™ TOPO™ PCR Cloning Kits offers the fastest and easiest method for high-efficiency (cloning of blunt-end PCR products amplified with proofreading thermostable polymerases. The kits include linearized … WebLimited Label License for StrataClone Blunt PCR Cloning Kits US Patent No. 7,109,178 and patents pending. Purchase of this product conveys to the purchaser only the non-transferable right under these patents to use the product for research use only by the purchaser. No rights are granted to the purchaser エクセル wbs 日付 https://boxh.net

关于ELISA试剂盒的网上超市合同公告-浙江机电设备采购网

WebThis PCR Cloning Kit contains an optimized Cloning Mix containing a proprietary ligation enhancer and a linearized vector that uses a novel mechanism for background colony suppression to give a low background. Web平末端载体pEASY－blunt ZERO Cloning Kit和感受态细胞Trans1－T1Chemically Competent Cell（购自全式金公司）。互补载体pCAMBIA 1301，过表达载体pCAMBIA 130135SN。 1.2 方法. 1.2.1 拟南芥植株培养 WebOct 1, 2016 · Features of the pEASY-Blunt Zero Cloning Kit 5 minutes fast insertion of blunt-end PCR products.by topoisomerase. High cloning … palm medical llc hialeah

pEASY-Blunt Zero (linearized) Sequence and Map - SnapGene

Category:Magen Biotech- Expert in Biological Sample Preparation (from 2012)

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Peasy庐-blunt zero cloning kit

pEASY® -Blunt Cloning Kit - TransGen Biotech

WebThe Zero Blunt™ TOPO™ PCR Cloning Kit for direct insertion of blunt-ended PCR products into a plasmid vector can be purchased with a variety of competent cells that deliver different advantages depending upon your needs: General cloning: TOP10 cells (Cat. No. K2800-20, K2800-40) WebJul 9, 2024 · （3 ） 常用试剂和引物 pEASY-Blunt Zero Cloning Kit 购自于北京全式金生物技术有限公司，植物RNA 小 量提取试剂盒购自上海莱枫生物科技有限公司；Pfu DNA 聚合酶、所有限制性内切酶、 RNase A 、Taq DNA 聚合酶、T4 DNA 连接酶、质粒小提试剂盒、DNA 分子量标志、DNA 凝胶 ...

Peasy庐-blunt zero cloning kit

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WebpEASY® Blunt Zero Cloning Vector contains a suicide gene. Ligation of PCR fragment disrupts the expression of the gene. Cells that contain nonrecombinant vector are killed … WebZero Blunt ® TOPO ® PCR Cloning Kits are shipped on dry ice. Kits supplied with competent cells containa box with Zero Blunt ® TOPO ® PCR Cloning reagents (Box 1) and a box with One Shot ® E. coli (Box 2). Zero Blunt ® TOPO ® PCR Cloning Kit supplied with the PureLink ® Quick Plasmid Miniprep Kit (Cat. no.K2800-02) is shipped with an ...

WebHiPure Circulating DNA Kit IVD3182 Isolation circulating DNA from 1-5ml plasma, serum, body fluids using vacuum protocol HiPure Blood DNA Mini Kit D3111 Isolation total DNA from 200μl whole blood News View more Magen helps the success of Cell-free DNA Research and Application Magen helps the success of Cell-free DNA Research and … WebFeatures of the pEASY-Uni Seamless Cloning and Assembly Kit. Fast assembly and fast cloning: 15 minutes. Easy: regardless of restriction enzyme sites of inserts, and elimination of restriction digestion of inserts. High efficiency: up to 95% positive clones. Seamless Assembly: no extra sequences introduced.

WebThe “pEASY ” cloning vectors provide a highly efficient, 5 minutes, ... Cloning kits are supplied with free Trans1-T1 Phage Chemically Comptent Cell. Publications with pEASY vector Applications: > Sequencing; > ... pEASY®-Blunt Zero p (3956 bp) U C o ri l a c Z A mp ic l n K a n a m y c i n WebProduct Details pEASY® - Blunt3 Cloning Vector provides dual EcoR I and dual Not I enzyme digestion sites. It is designed for cloning and sequencing Pfu-amplified PCR products. The cloned insert can be released from a single enzyme digestion. •5 minutes fast ligation of Pfu-amplified PCR products. •Ampicillin resistance gene for selection.

WebZero™ Blunt™ TOPO™ kits are available with a variety of competent cells, or without competent cells, depending on your needs and budget. Features of the Zero™ Blunt™ TOPO™ PCR Cloning Kits: Fast and easy— go from PCR to clones in just 3 steps and in as little as 5 minutes hands-on time. Efficient— obtain up to 95% clones with ...

WebProduct Details pEASY® -T5 Zero Cloning Vector contains a suicide gene. Ligation of PCR fragment disrupts the expression of the gene. Cells that contain non-recombinant vector are killed upon plating. Therefore, blue/white screening is not required. •5 minutes fast ligation of Taq-amplified PCR products. •High cloning efficiency. palm memorial sierra chapel oakhurst caエクセル webWebFeb 15, 2024 · 分别以杜梨cDNA和gDNA为模板扩增PbGID1家族基因。扩增程序为：95 ℃预变性2 min；95 ℃ 30 s，55 ℃ 30 s，72 ℃ 1 min，30个循环；72 ℃ 延伸 2 min。PCR产物进行胶回收后，连接至pEASY-Blunt Zero Cloning Kit克隆载体，转化大肠杆菌感受态DH5α中，挑取单克隆测序。 palm-mallWebpEASY® -Blunt Cloning Kit Catalog Number：CB101-01 Price：$89.02 Specification： 20 rxns 60 rxns Quantity： Manual Vector Information MSDS COA Inquire Now Buy Now Add … palmmicro communicationsWebProkaryotic expression vector for cloning blunt-ended PCR products and adding an N-terminal 6xHis tag. ... pEASY-Blunt Zero (linearized) pJAZZ-OC. pSMART HCKan (linearized) phiX174. pEASY-Blunt3 (linearized) pJAZZ-OK. Plasmid Sets. Basic Cloning Vectors. I.M.A.G.E. Consortium Plasmids. palmmicro ar1688Web一、 采购人名称： 石河子大学 二、 供应商名称： 石河子市北泉镇诺泰实验用品经销部 三、 采购项目名称： 石河子大学网上超市项目 四、 采购项目编号： ***** 五、 合同编号： 11n ***** 六、 合同内容： palm memorial funeral home chowchillaWebpEASY-Blunt (linearized) Cloning vector for blunt-ended PCR products, with blue-white selection, kanamycin and ampicillin resistance markers, and a T7 promoter. Sequence Author: TransBionovo (TransGen Biotech) Open in SnapGene Try SnapGene for Free Download Plasmid Download SnapGene Viewer エクセルwebクエリ