WebTOPflash (TCF Reporter Plasmid) Transfection grade T-cell factor reporter plasmid containing 2 sets (with the second set in the reverse orientation) of 3 copies of the TCF binding site (wild type) upstream of the Thymidine Kinase minimal promoter and Luciferase open reading frame. - Find MSDS or SDS, a COA, data sheets and more information. WebFrom what I can understand, TOPflash is a luciferase reporter plasmid that contains two sets of 3 copies of the wild-type TCF binding regions. If the canonical Wnt signaling is activated, beta-catenin will translocate to the nucleus to associate with TCF/LEF …
TOP/FOP-FLASH assay in HEK293T cells?
Web29. jún 2024 · TOP/FOP-Flash luciferase reporter assay and western blot were used to analysis the activation of Wnt/β-catenin signaling pathway. Si-CASC11-transfected HeLa cells were subcutaneously inoculated into male athymic (nude) mice to investigate the effect of CASC11 on the tumor formation. Results: WebLuciferase assay HEK 293-TOP cells (1 x 104) or Jurkat-TOP/FOP (2 x 104) cells were seeded into 96-well plates and incubated in medium with 10% FBS for one day. The cells were then stimulated... other names for cooler
Experience with TOP FLASH ASSAY? ResearchGate
Web14. nov 2024 · The re-localization of β-catenin prompted us to investigate Wnt-dependent transcription by using the Dual-luciferase reporter system and TOP/FOP reporter assays system. Upon knock-down of H19, the TOP/FOP ratio decreased to about 50% when compared to the control in both MCF-7-R and SK-BR-3-R cells ... Web背景技术微小RNA（miRNA）在肿瘤的发生和发展中起着至关重要的作用。以前，我们表明miR-504被下调并抑制胶质母细胞瘤（GBM）中的肿瘤增殖。然而，miR-504在GBM间充质（ME）过渡中的调控及其相关机制仍不清楚。方法转录组和临床数据来自癌症基因组图谱（TCGA）数据库。 Web( A ) TOP/FOP luciferase reporter assays of 8988T cells treated with vitamin C (4 or 5 mM) for 24 h. ( B , C ) PDAC cells were treated with vitamin C (4 or 5 mM) for 24 h and immunoblotted with the indicated antibodies. Error bars reflect the s.d. of three … rock greatest hits youtube